《植物生理学报》 2016, 52(2): 177-186

盐胁迫下黄花补血草幼苗ROS代谢酶活性的变化

马婷, 滕玉瑾, 李翠祥, 杨颖丽^*

西北师范大学生命科学学院, 兰州730070

通信作者：杨颖丽；E-mail: yangyingli2006@sohu.com

摘　要：

用不同浓度NaCl (0、25、50、100和150 mmol•L^-1)处理盐生植物黄花补血草(Limonium aureum)幼苗, 探究幼苗叶片中抗氧化酶和多胺氧化酶活性等生理指标变化与其耐盐性的关系。研究表明: 黄花补血草幼苗叶片中羟自由基(•OH)含量仅在150 mmol•L^-1NaCl处理下明显增加, 而其他盐浓度均导致该指标降低; 不同的是, 高浓度NaCl (100和150 mmol•L^-1)处理下过氧化氢(H₂O₂)含量显著增加; 所有盐浓度均诱导幼苗叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、二胺氧化酶(DAO)和多胺氧化酶(PAO)活性增强; 盐胁迫使细胞壁结合态POD活性降低; 此外, 幼苗叶片中的过氧化氢酶(CAT)活性在低盐胁迫下增强, 高盐胁迫下受到抑制。以上结果说明黄花补血草幼苗可响应盐胁迫, 使幼苗叶片中抗氧化酶和多胺代谢酶活性增强, 且高盐诱导叶片中H₂O₂和•OH增加, 这可能与幼苗对盐环境的适应有关。

关键词：黄花补血草; 盐胁迫; 活性氧; 抗氧化酶

收稿：2015-10-16   修定：2016-01-06

资助：国家自然科学基金(31360094、31470464和31160088)。

Changes of ROS metabolizing enzyme activities in Limonium aureum seedlings under salinity stress

MA Ting, TENG Yu-Jin, LI Cui-Xiang, YANG Ying-Li^*

College of Life Sciences, Northwest Normal University. Lanzhou 730070, China

Corresponding author: YANG Ying-Li; E-mail: yangyingli2006@sohu.com

Abstract:

Halophyte Limonium aureum seedlings treated with different NaCl concentrations (0, 25, 50, 100 and 150 mmol•L^-1) were used to investigate the relationship between the changes of physiological indexes, such as the activities of antioxidant enzymes and polyamine metabolizing enzymes, and salt tolerance. Except for increased hydroxy radical (•OH) content due to 150 mmol•L^-1 NaCl, different salinity concentrations resulted in the reduction of this paraneter in the leaves. Differently, hydrogen peroxide (H₂O₂) content significantly rose in response to 100 and 150 mmol•L^-1 NaCl. Further study showed that all salinity concentrations stimulated superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), diamine oxidase (DAO) and polyamine oxidase (PAO) activities, but inhibited cell wall-bound POD activity in the leaves of L. aureum seedling. Additionally, catalase (CAT) activity rose due to low salinity concentrations but lowered to high NaCl concentrations in the leaves. Taken together, L. aureum seedlings could respond to salt stress and exhibited the increases of the activities of antioxidant enzymes and polyamine metabolizing enzymes, and high salinity stimulated H₂O₂ and •OH generation, which might be associated with the adaptation of the seedlings to salt environment.

Key words: Limonium aureum; salinity stress; reactive oxygen species; antioxidant enzymes